“Function and metabolic profile of platelets on the experimental infection by Achromobacter xylosoxidans”

Platelets are anucleate blood elements that have considerable role in modulating hemostasis. Recent studies indicate that platelets are essential regulators of the immune system and exhibit a fundamental role in infections. Recently, we have characterized lung experimental acute infection by Achromobacter xylosoxidans, in which we showed the essential function of the innate immune system for infection control. Therefore, we hypothesized that platelets contribute to host response against lung infections by bacteria. In this work, we propose to use classical immunological techniques combined with strategies of systems biology (lipidomics and metabolomics) that will enable us to evaluate: 1) platelet interaction and antimicrobial activities against A. xylosoxidans; 2) platelet activation and production of mediators in response to bacteria and 3) identify the metabolomic profile of the interaction between platelets and A. xylosoxidans and associations with produced inflammatory mediators.Accordingly,we will demonstrate that platelets exert a significant role during A. xylosoxidans infection.