Nanoplataform based in ruthenium nitrosyl and fluorescent Carbon dot from gelatin

Carbon dots (CD) are nanoscale semiconductor particles and have light emission properties. They can be obtained from different lower cost carbon sources such as organic waste1. Due to its luminescence, biocompatibility and higher water solubility this material has been developed for biological applications, specifically in bioimaging. This work showed the fluorescence CD from commercial gelatin based hydrothermal method using a domestic microwave and fluorescent properties with [Ru(NH.NHq)(tpy)NO]3+ (RuII-NO+) complex2. After purification by dialysis bag, the CD exhibit fluorescent emission at 400 nm and fluorescence stability in aqueous solution (pH=7) during 30 days under 5 0C. Fluorescence quenching studies showed that CD and RuNO exhibit electrostatic interaction between negative charge from CD (δ = ̶ 20 mV) and positively charged group as nitrosyl (NO+) ligand of the ruthenium complex. The nanoplataform CD-/+NO-RuII under biological reducer (noradrenaline) enable the nitric oxide (NO0) release from RuNO as well as oxidation of CD which now exhibits fluorescence emission in the visible region (500 nm).