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A. comosus L. Merril, popularly known as pineapple, is the best known species of the family Bromeliaceae, and also its only extensively cultivated food source. In 2018, Brazil represented the second place in world production of pineapple, behind only Costa Rica. Several parts of pineapple, such as leaves, green fruits, cortex, bark and stems1, are used for the extraction of metabolites with ethanol2 and methanol3. A great number of studies related to A. comosus focus on pharmacologic purposes (anti-inflammatory, antitumor, antioxidant and antiviral)4. Besides many studies carried out to identify metabolites, there are just a few of them regarding the optimization of extraction conditions in relation with the plant secondary metabolite profile5,6. Thus, the main goal of the present work was to develop an extraction method which was able to gather a greater diversity metabolites of A. comosus leaves, using as extractor solvents ethanol, methanol and water in different concentrations, it was also analyzed different times ultrasound bath to maximize the extraction. The validation of the extraction method was analyzed from the metabolic profile applied to UHPLC-DAD-FTHRMS. With help of the literature review and extraction analyzes performed by the author, some results have already been achieved and showed that, in terms of total mass yield, methanol with water in 8: 2 and 9:1 proportions are more efficient for metabolite extraction. However, studies of the extracts’ metabolomic profilling will validate which method is more efficient, based on the greater variability of the substances.
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