Development and validation of an ecological IR spectrophotometric method for quantification of vancomycin dosage form

Vancomycin, an antimicrobial, does not present a quantitative method in the literature by spectrophotometry in the infrared region for the evaluation of its pharmaceutical product. So, the aim of this work was to develop and validate a new, ecological, low cost and fast infrared spectrophotometric method using potassium bromide (KBr) and the band between 1450-1375 cm-1. It was linear in the range of 1.0–2.0 µg/150 mg, with correlation coefficient of 0.9994. Selective when the spectra of vancomycin reference and sample were compared. Precise by repeatability and intermediate precision with RSD of 1.67% and 3.12%, respectively. Accurate with mean recovery of 99.37% and robust when strength and compression time of the pellets and KBr brand were varied. The method can be applied in the routine analysis of vancomycin dosage form and is an important alternative for the current and sustainable pharmaceutical analysis.