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Scaffolds are three-dimensional and porous structures that mimic specific biological, mechanical and physical characteristics of the extracellular matrix of the target tissue [1]. The aim of this study was to develop dense lamellar scaffold (DLS) and to evaluate its physical-chemical and morphological properties. The lamellar structure of DLS was obtained using polymeric mixture of collagen, silk fibroin, polyethylene glycol 400 and polysaccharides extracted from peel of grape. The characterization was accomplished by FTIR, µCT, SEM and surface zeta potential analyze to evaluate structural properties, surface morphology, porosity and pore interconnectivity. The DLS had open pores, interconnected with pore size between 100-160 µm. Surface zeta potential was a function of pH (8 to 4), -4.98 mV (pH 8.40) and -1.90 mV (pH 3.88), respectively. FTIR showed that chemical interactions occurred between DLS components. Finally, the results indicate that DLS has potential for complementary studies aiming at biomedical applications.
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