Computational Fluid Dynamics Analysis of Transport Phenomena in a Bone-on-Chip Platform for Leukemia-Related Bone Alterations

Bone-on-chip (BoC) devices are promising in vitro platforms for reproducing key aspects of the bone tissue microenvironment under controlled conditions. In this study, Computational Fluid Dynamics (CFD) were applied to investigate laminar flow and coupled convective–diffusive transport in a microfluidic system designed to study leukemia-related bone alterations. The model enables the quantification of velocity fields, pressure distribution, wall shear stress, and concentration profiles, which directly affect the species distribution within the device. From an engineering perspective, this study establishes relationships between device geometry, flow conditions, and transport performance, providing quantitative disease-oriented criteria for the optimization of BoC platforms.