Biological Evaluation of BODIPY-Functionalized Mn(I) Complexes as Dual PhotoCORM and Photosensitizer Agents for Cancer Therapy

Photodynamic therapy (PDT) has emerged as a promising strategy for cancer treatment by combining light activation with the selective generation of reactive oxygen species. The therapeutic potential of PDT can be further enhanced through the incorporation of light-activated carbon monoxide-releasing molecules (PhotoCORMs), enabling dual-action therapeutic systems. In this work, a series of BODIPY-functionalized Mn(I) tricarbonyl complexes were developed and evaluated as potential photosensitizers and PhotoCORMs. Their photophysical and photochemical properties were investigated, together with their ability to release CO and generate singlet oxygen under visible-light irradiation. Furthermore, their biological applicability was explored through fluorescence microscopy in healthy and cancer cell lines, demonstrating efficient cellular uptake and preliminary evidence of mitochondrial association. Ongoing biological studies aim to evaluate their phototoxicity and elucidate their mechanism of action, supporting their potential as multifunctional phototherapeutic agents.