Results from studies of floral emissions of more than 40 different tropical flowers will be presented. These emanations were monitored according to the time of day and part of the flower (petals, pistils, etc.), detecting more than 200 different volatile compounds, mainly, attractants of pollinating insects or those insects that are natural enemies of predators. Flowers attract pollinators through their exotic forms and multiple and vivid colours. The study of colours of tropical flowers is related to extraction with solvents (methanol, ethanol, water) or by matrix solid-phase dispersion (MSPD), of anthocyanins, flavonoids or other phenolic compounds. Liquid chromatography coupled to mass spectrometry (LC-MS) was used to analyse these complex mixtures. Examples of more than 20 tropical flowers of distinct colours, whose hydro-alcoholic extracts were analysed by LC-MS with electrospray interface, using different high-resolution mass analysers (HR-TOF, Orbitrap), will be presented.