These instructions are an example of what a properly prepared meeting abstract should look like Trichilia catigua A. Juss. (Meliaceae), known as ‘catuaba’ and ‘catiguá’, is a plant widely found in South America to Central America. The barks are mainly used as stimulants; and have antioxidant, analgesic, vasodilator, anti-inflammatory and anti-depressant action, and is also used in folk medicine as a tonic for the treatment of fatigue, stress, impotence and memory deficits. At the chemical point, the species stands out for having a high content of phenolic compounds, including flavonoids and tannins. Considering the scientific and economic interest that this kind awakens, it is essential to conduct studies aimed at the isolation and structural identification of substances present in the semipurified fraction. In this study, ethyl-acetate-fraction obtained from the crude extract acetone:water (7:3, v/v) from T. catigua barks was subjected to various chromatographic methods, such as column chromatography, high speed countercurrent (HSCCC), and thin-layer chromatography with the aim of isolate phenolic compounds. Identification was performed by spectroscopic methods of nuclear magnetic resonance (NMR) 1D (1H and 13C), 2D NMR (1H/1H-COSY), and mass spectrometry. According the isolation, it was identified the following compounds: neoclorogenic acid (1), epicatechin (2), and cinchonains Ia (3), Ib (4) and IIb (5).