Phytochemical Study of Cespedesia spathulata.

Débora Ramos de Oliveira, Elisa Magno de Almeida, Mariana Rodrigues da Silva, Ana Luisa Bento da Silva Barbosa, Raimundo Braz Filho, Mario Geraldo de Carvalho.
Departamento de Química ,Instituto de Ciências Exatas (ICE), UFRRJ.
UFRRJ, BR 465, Km-7, Seropédica CEP: 23890-000.
[email protected]

The genus Cespedesia belongs to the Ochnacea family, Which has been known as a bioproducer of flavonoids and biflavonoids (FIDELIS, 2014). The revision of the genus proposed by SASTRE (1995), synonimized the eight described species of the genus and the three combinations made to a single species: Cespedesia spathulata, making it a monospecific genus. Cespedesia spathulata is recognized for its spatulate leaves with obovis and lax inflorescences (CHACON 2011). This work reveals the results of isolation and identification of special metabolites in the extracts from C. spathulata. Leaves of the plant were collected in September 2016 at the Botanical Garden of Rio de Janeiro. After drying and powdered were subjected to extraction by cold maceration with hexane and hydromethanolic solution. The hydromethanolic extract (242.2 g) was partitioned using hexane (4.82 g), dichloromethane (3.52 g), ethyl acetate (32.84 g) and butanol (22.32 g). Partitioning of the hexane, dichloromethane and ethyl acetate fractions was done by chromatographic techniques as the open silica gel column, sephadex LH20 and HPLC. Fractions were compared by analytical thin-layer chromatography and the ones of interest were analyzed with 1H and 13C NMR, GC-MS. Spectra analysis and comparison with literature data led to the identification of a series of fatty methyl esters, phytol and mixture of sitosterol with stgmasterol in the fraction obtained in the hexane partition. The biflavonoid ochnaflavone (SON et al., 1992) was identified in fractions of the dichloromethane partition. The flavonoids 6''-O-acetyl-vitexin (HO & KIM, 1993), catechin and epicatechin (LU & FOO, 1999) were identified in the fraction of ethyl acetate. These are the first results of phytochemicas in this genus and are in agreement with the bioproducts identified in species of the Ochnacea family.
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