Azadirachta indica A. Juss. (Meliaceae) is commonly known as neem, originating in Asia and already found in other continents. Neem has been widely used by humankind to treat diseases since prehistory1. Is one of the major bioproducers of limonoids, which constitute 1/3 of its chemical composition. Biological activities such as antibacterial, antiviral, antifungal, molluscicide and antihyperglycemic have been attributed to different parts and extracts of neem, besides the antifeeding activity characteristic of limonoids present in this species, especially Azadirachtin2,3. The phytochemical study of A. indica A. Juss roots were carried out using classical chromatographic methods for the isolation of compounds present in the methanolic extract / partition dichloromethane and ethyl acetate. The A. indica roots were collected in Cachoeiro de Itapemirim city, Espírito Santo state, Brazil, in December 2015. The structures were elucidated by uni and bidimensional Nuclear Magnetic Resonance (NMR 1H and 13C), High Resolution Mass Spectrometry (HRESI-MS) and Low Resolution Mass Spectrometry (LR-MS). The chemical investigation of A. indica roots partition dichloromethane has resulted in the identification of four known limonoids: Nimbinene4, nimbandiol4 nimbinal5 and salannol-3-acetate5, and ethyl acetate partition culminated a new structure named morenolide (1). Compound 1 is similar to compound 26, differing by the presence of the acetyl group on carbon atom C-6. The HRESI-MS of compound 1 showed the peak corresponding to the molecular ion (m/z 503.2033 [M+Na]+, C28H32NaO7, calc. m/z 503.2046).