Isatin is a synthetically versatile, indole compound used for the synthesis of a variety of heterocyclic compounds. Studies have shown that it, as well as its derivatives, has several activities, among them the herbicide. One of the main targets of the available herbicides is the enzyme Acetohydroxyacid Synthase, exclusive of plants and some microorganisms. However, due to the great use of these products, weed strains have become resistant, generating problems in many areas. Thus, it is necessary that new inhibitory structures be developed. Evaluation of inhibition of seed germination in the Lactuca sativa of isatin derivatives obtained by N-alkylation and azide-alkyne cycloaddition reactions. The experiment was conducted in DIC with seven treatments, five replicates per treatment. The repetition was consisted of one plate with 20 seeds and 5 ml of 100 µM solution per treatment, whereas the control was treated with 5 ml of distilled water. After 15 days, seed viability analyzes were performed, such as percentage of germination (G%) and germination speed index (GSI). When compared to the control group, there was no difference between treatments for G%. On the other hand, the compounds 1-(4-nitrobenzyl)indoline-2,3-dione (3) and 1-((1-(4-bromophenyl)-1H-1,2,3-triazol-4-yl)methyl)indoline-2,3-dione (5) statistically reduced GSI. Further tests should be performed, including determination of aereal length and mechanisms of action that explain the ratios of (3) and (5) to reduce GSI.