In chemistry of natural products, the discovery of new or bioactive compounds is the one of the main challenges. In this sense, Dereplication strategies contribute in search of interest compounds quickly since it is possible the previous identification of the compounds before the isolation. In this work, by LC-HRMS the crude hexane fraction of the stems extract of Swinglea glutinosa (Rutaceae) was analyzed. As complementary tool was used the software Target analysis from Bruker Daltonics; the screening software was connected with an internal compound database, containing compounds that have been previously reported in this specie, via an in-house-built Excel application that generated automatic search lists for Target Analysis, and made it possible to search for the most likely adduct and/or fragment ions. This approach allows screening extracts from many different plants or microorganisms and easily identifying chromatographic peaks that do not correspond to known compounds allowing rapid determination of novel compounds. The Figure 1 shows the chromatogram obtained by LC-HRMS containing unknowns (the colorless one) and known compounds (the colored one) in the analyzed fraction. The stem hexane fraction, that was active against Human Leukemia Cells, was submitted to preparative HPLC procedure for the isolation of an unrelated amide in the genus, Lansiumamide B (N-methyl-N-cis-styrylcinnamamide). Thereafter, the compound will be submitted to the Leukemia assays.