Cerrado is a biodiversity hotspot undergoing massive anthropogenic transformation. This biome is famous for its high endemism, and Brazilian eupatoriae species (Asteraceae family) are prevalent with 440 species and 40 genera endemic to the country. Among them, one specie is peculiar, Lomatozoma artemisiifolia Baker, since it is endemic from Cerrado and it is found only in the Goiás state, Brazil (Trindade et al, 2014). Additionally, there is no literature about its chemical or biological study.
Species from Eupatoriae tribe presents several class of compounds such as flavonoids and sesquiterpenic lactones (Taleb-Contini, et al, 2007; Salapovic et al. 2013).
The leaves of L. artemisiifolia were collected on February 2016 in Parque Estadual da Serra Dourada, localized in Mossâmedes (GO) and were identified by Dr. Aristônio Magalhães Teles. The fresh leaves were extracted with EtOAc, the solvent was eliminated at 40°C under vacuum with rotatory evaporator to afford AcOEt extract (EA1). EA1 was submitted to chromatographic column separation (silica gel 60) wiht eluent system: hexane and ethyl acetate (100:0 to 50:50) to afford 33 fractions. After successive chromatographic procedures, two of these subfractions AM1 and AM2 were analysed by spectroscopic methods.
The NMR 1H data of AM2 compound shows shifts at  7.73 (1H, d, 1.8 Hz), 7.64 (1H, dd, 8.5 and 1.8 Hz), 7.04 (1H, d, 8.5 Hz), 6.75 (1H, s) and singlets (3H each) at  4.02, 3.98, 3.97, 3.92 and 3.86 ppm which are characteristic of aromatic protons from A and B ring flavonoid skeleton with five methoxy substituents.
Mass spectra of the fractions (AM1 and AM2) were acquired using the high resolution and high accuracy orbitrap instrument coupled with a Dionex, Ultimate 3000, UHPLC focused, that allowed us to confirm the presence mainly of hydroxi-methoxy flavonones with m/z 345.06223 (C17H14O8); m/z 359.07797 (C18H16O8) and m/z 387.10833 (C20H20O8). The further examination of the fragmentation profile by ESI(-)-MS/MS will be able to determine the exact isomer present in this fractions.
This is the first phytochemical study of L. artemisiifolia. Another aim of this work is to obtain an efficient protocol to establish this species on in vitro culture. Seeds were submitted to superficial disinfestation by immersion in 2.5% NaClO solution and placed in MS culture medium with 30 g.L-1 sucrose and 7 g.L-1 of agar, under aseptic conditions. Optimum culture condition for germination were determined and multiple shoots were generated. The next step will be the comparison by LC-HRMS profile of AcOEt extract from plants propagated in vitro and those harvest in nature.

References:
Taleb-Contini, S.H. et al . 2007, Detection of flavonoids in glandular trichomes of Chromolaena species (Eupatorieae, Asteraceae) by reversed-phase high-performance liquid chromatography. Rev. Bras. Cienc. Farm., São Paulo , 43 (2), 315-321.
Trindade, L.M.P; Fernandes, Y.S & Gonçalves, L. A. de, 2014 Diversidade e desenvolvimento dos tricomas glandulares de Lomatozona artemissifolia Baker, uma espécie endêmica do estado de Goiás, IHERINGIA, Sér. Bot., Porto Alegre, v. 69, n. 2, p. 235-243.
Salapovic, H.; Geier, J.; Reznicek, G. 2013 Quantification of Sesquiterpene Lactones in Asteraceae Plant Extracts: Evaluation of their Allergenic Potential. Sci Pharm, 81, 807–818.

Acknowledgement: CRTI for LC-HRMS analysis and Fapeg, Capes and CNPq for financial support.