Flavonoids isolated from Anomalocalyx ulenus leaves (Euphorbiaceae)

The plants of the Euphorbiaceae family have great chemical diversity and are characterized by their medicinal properties. Anomalocalyx uleanus is a unique species of the genus Anomalocalyx, belonging to the family Euphorbiaceae, of restricted and exclusive occurrence in the Brazilian Amazon, and is found mainly in flooded areas. Despite being a unique species, A. uleans is of great importance due to the complexity of phytogeographic and taxonomic nature of the Euphorbiaceaes that need to be better elucidated. Therefore, the present work aimed at performing a phytochemical study of the present metabolites in the ethyl acetate partition of A. uleanus leaves. The botanical material was collected from the Jarú Biological Reserve, located in Ji-Paraná-RO, Brazil. The leaves were oven dried and ground, the crude extract was extracted with methanol/water (7:3). Then the liquid-liquid partition process was performed to obtain the ethyl acetate partition. This partition was subjected to molecular exclusion column chromatography using Sephadex LH-20 as stationary phase. Through analysis of thin layer chromatography (TLC), four fractions were selected, reunited and subjected to analysis by High Performance Liquid Chromatography (HPLC). At the end, four flavonoids were isolated and elucidated by Nuclear Magnetic Resonance, 1H NMR: astilbine, catechin, epicatechin and quercetin-3-O-α-L-ramnopyranoside.