Wounds and skin abscesses are common in the clinical routine of dogs and cats and are usually due to surgical wounds, bites by other animals or running over. Because of that, the aim of this study was to investigate the cases of cutaneous lesions in dogs and cats between August 2004 to December 2015, in order to identify the main bacterial species and their antimicrobial resistance profile, which affected the animals treated at the Veterinary Clinical Hospital (HCV) UDESC. This study showed an increase in the number of tests ordered over the years, where the number of samples between the years 2004-2011 corresponded to only 18.7% (29/156), while the year 2015 alone amounted to 32.7% (51/156). A total of 156 samples, 38 (24.4%) showed no growth, 91 (58.3%) isolates were Gram-positive bacteria and 27 (17.3%) Gram-negative, where the main isolated microorganism was Staphylococcus sp. with 61% (72/118), followed by Streptococcus sp. (15.25%) and Escherichia coli (8.47%). Other microorganisms isolated were: Citrobacter sp.p, Enterobacter spp., Proteus sp. and Pseudomonas aeruginosa with 1.9% each, Pasteurella sp. and Klebsiella sp. with 1.3% and Corynebacterium sp. and Acinetobacter baumannii with 0.6%. A total of 72 Staphylococcus, 32 were tested for oxacillin, antimicrobial of choice for phenotypic isolation methicillin-resistant Staphylococcus (MRS), 56.25% of these were resistant and in such cases the use of antimicrobials, as beta-lactam antibiotics are not indicated. Penicillin tested only on some Gram-positive samples showed 55.56% (40/72) of resistance, tetracycline 49.23% (32/65) and doxycycline 35.1% (26/74), while norfloxacin showed 23.94% (17/71). These 4 antimicrobials were the most used over the 12 years. Currently, the main antimicrobials used in abscess samples and skin lesions in HVC are: cephalexin, gentamicin, neomycin, ciprofloxacin, enrofloxacin and amoxicillin with clavulanate, that showed resistance of 46% (23/50), 15.1% (8/53), 35.7% (20/56), 30.8% (8/26), 24.6% (15/61) and 30.2% (13/43), respectively. Over the years, the discovery of multiresistant microorganisms and the use of diagnostic techniques in microbiology obtained greater importance, including in veterinary medicine, helping to establish the resistance profile of a veterinary hospital in order to keep an epidemiological surveillance and educate about the appropriate use of antimicrobials.