In Vitro ANTIMICROBIAL CAPACITY OF Pelargonium graveolens EXTRACTS AGAINST BACTERIA THAT CAUSE ACNE VULGARIS
Studies using plants have been promising in the research for new medications. One of the lines of research is antimicrobials, which currently have been suffering with the resistance of many pathogens responsible for diseases. Since the plant Pelargonium graveolens has antimicrobials properties, and being acne a pathology that affects thousands of people around the world, the objective of this research was to evaluate the in vitro capacity of extracts of this plant in inhibiting bacteria that cause acne vulgaris, such as Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. Plant extracts were prepared using five different solvents: ethanol, ethanol 50%, ethanol 70%, methanol and acetone; concentrations of 25, 50 and 100uL of the extracts over bacterial cultures suspended in nutritive broth and soy triptych 5% were tested. Every sample was incubated for 24 to 48 hours, and then analyzed on the spectrophotometer at 546 nm absorbance. The presence of terpenes was evaluated by chromatography on the thin layer. The results showed inhibition of the growth of three organisms with acetone extract, with a maximum inhibition of 27% for S. aureus, 56% for S. epidermidis and 82% for P. acnes. Regarding the inhibition relevance, acetone was the most significant extract of the inhibited samples. The presence of terpenes was verified by the appearance of a violet spot in the acetone extract, with Rf 0,75 related with the antimicrobial capacity described. The toxicity and safety of this plant must still be studied and therefore is not available yet for human consume.