Candida tropicalis has progressively become one of the main human fungal pathogen; however, the studies about its virulence are limited. Biofilm formation and filamentation potential are important virulence factor for this species. The present study evaluated the effect of phenotypic switching on biofilm formation and assessed filamentous growth of planktonic and sessile cells recovered from biofilms formed by distinct morphotypes of C. tropicalis that arose phenotypic switching. To evaluate the influence of phenotypic switching on biofilm formation, were evaluated four different switching systems, including (49.07, 46.10. 522.11 and 700.11). For two of the switching systems tested (related to isolates 49.07 and 46.10) the morphotypes (parental, switch variants and revertants) exhibited variable biofilm formation; differently, morphotypes of the other two switching systems tested (related to isolates 522.11 and 700.11) did not exhibit differences in the amount of biofilm formation. To evaluate the potential of filamentation, sessile cells and planktonic cells were recovered from biofilms formed by morphotypes of two switching systems (49.07 and 700.11). Differentiation in filamentous forms occurred among both planktonic and sessile cells recovered from biofilms of all morphotypes tested. For the majority of morphotypes, none differences were observed regarding filamentous growth between planktonic cells and sessile cells; except for the revertant of a crepe variant (49.07) where highest filamentous growth occurred among sessile cells. Filamentation among sessile cells of the variant rough (49.07) was higher than that observed for its parental counterpart strain. Concerning planktonic cells, none differences in filamentation between either switch strains or revertants with their parental strains were observed. This is the first study that compares the potential of filamentation between sessile and planktonic cells recovered from biofilms formed by switching phenotypic derived strains.