The growing concern over the presence of bacterial strains resistant to antimicrobials in food, stands out because of the possibility of resistance transmission to humans. Thus, the aim of this study was to evaluate the resistance profile of coagulase positive Staphylococcus (CPS) and coagulase negative Staphylococcus (CNS) strains, obtained from milk and Serrano Handmade Cheese in 2013 of the mountain region of Santa Catarina. The data belonged to the database of Microbiological Animal Diagnostic Center (CEDIMA) at Santa Catarina State University. A hundred and eight strains (59 CPS and 47 CNS) were purified and subjected to antimicrobial susceptibility testing according to the Clinical and Laboratory Standards Institute (CLSI), with the following antimicrobials: oxacillin, cefoxetin, penicillin, amoxicillin, ampicillin, amoxicillin + clavulanate, cephalothin, cephalexin, ceftriaxone, imipenem, meropenem, amikacin, neomycin, streptomycin, tobramycin, gentamicin, enrofloxacin, norfloxacin, ciprofloxacin, levofloxacin, azithromycin, erythromycin, clindamycin, doxycycline, tetracycline, rifampicin, trimethoprim-sulfamethoxazole. A total of 20.75% (15 CPS and 7 CNS) of 106 strains showed no resistance to the tested antimicrobials. The highest resistance rates were penicillin 34.90% (23 CPS and 14 CNS), oxacillin 34% (23 CPS and 13 CNS), amoxicillin 18.86% (14 CPS and 6 CNS), erythromycin 15% (15 CPS and 1 CNS) and cephalothin 14.15% (13 CPS and 2 CNS). The coagulase positive strains have higher resistance index compared to coagulase negative. Among the CPS the lowest resistance rates were doxycycline, enrofloxacin, ciprofloxacin, levofloxacin and imipenem (about 1% each). As expected the class of beta-lactam showed the greatest resistance to both CNS and CPS. The oxacillin is the antimicrobial of choice for phenotypic identification of methicillin-resistant (MRS) and presented 34% of resistance in this study. These indexes are below the values found in strains obtained from clinical human samples, where Staphylococcus aureus multiresistant stand out as an obstacle to the success of treatments. However, the presence of some resistance profiles is a serious problem because they are foodborne bacteria.