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Abstract

Staphylococcus sp. can be present as a component of the normal microbiota of different human sites. The nasal cavity is a common site for S. aureus colonization and a source of dissemination by direct or indirect contact. In particular, colonized healthcare professionals are potential source of S. aureus dissemination as they often touch equipment and manage patients. We collected a nasal swab from nursing students who never had any contact or developed any work inside the hospital environment. We also
collected blood samples for biochemical and hematological analysis; and asked questions about diseases, lifestyle, and addictions. This research was approval via Plataforma Brasil Protocol number: 05667319.7.0000.5515. Nasal samples were transported in Tryptic Soy Broth and immediately spread onto a Mannitol plate agar for bacteria isolation; the DNAse agar and resistance for Novobiocin and Polymixin B assays were used for phenotypic identification of Staphylococcus sp. In addition, Staphylococcus sp. Was
classified as coagulase-positive and coagulase-negative by coagulase test tube and by detecting the coa gene by PCR. The disk diffusion method was made for antibiotic
susceptibility profile analysis following the Clinical & Laboratory Standards Institute (CLSI). The hematological parameters via Sysmex XS-1000i, then manually reading slides; and biochemical parameters (triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and total cholesterol using Labtest kits and Labmax Plenno equipment were also analyzed. Nineteen students participated in this research which 15 (78.9%) were female and 4 (21.1%) were male. The highest frequency was 18 - 19 years old people (63%), one smoker (5.3%), five drink alcoholic drinks at least three times per week (26,5%), eight practice exercise (42.4%), and 11 presented some respiratory disease (58.3%). Alterations in blood lipids were present in 47.7% of people in at least one parameter. All participants presented S. aureus in the nasal cavity, without specific host characteristics related. About the resistance, none of the isolates were multidrug-resistant. The natural presence of S. aureus in the nasal cavity of nursing students can be a risk factor for bacterial dissemination in the hospital and other healthcare settings. In conclusion, it is essential to monitor healthcare students regarding colonization by S. aureus as a measure to control contamination and dissemination of this bacterium in healthcare environments.

Institutions
  • 1 Departamento de Microbiologia / Centro de Ciências Biológicas / Universidade Estadual de Londrina
  • 2 Departamento de Patologia, Análises Clínicas e Toxicológicas, Universidade Estadual de Londrina
  • 3 Departamento de Microbiologia e Imunologia / Universidade do Oeste Paulista
  • 4 Departamento de Patologia / Análises Clínicas e Toxicológicas / Universidade Estadual de Londrina
  • 5 Centro de Ciências Biológicas / Universidade Estadual de Londrina
Track
  • Clinical Microbiology
Keywords
Microbiota
heathcare students
nasal cavity