ISOLATION OF MICROORGANISMS FROM MARINE SAMPLES FROM PARANÁ AND SANTA CATARINA COAST

The ocean represents a promising habitat in the search for new microbial taxons which may have the capacity to produce enzymes for industrial interest, different from those produced by their terrestrial partners. Microorganisms derived from the marine environment have attracted attention due to their large amount of biologically active secondary metabolites as well as enzymes with biotechnological potential. The activity and metabolic potential of microorganisms depend directly on the conditions of isolation and correct preservation of microorganisms, being those fundamental stages for a successful prospection of compounds. In this way, the goal of the present work was the isolation of microorganisms associated to marine samples including coral, sea urchin, algae, barnacles and biofilm. The samples were collected in Bombinhas beach and Matinhos beach on summer of 2015 and 2016/2017 in Santa Catarina and Paraná States, respectively. For the isolation of filamentous fungi, marine samples were cut in 3 pieces and placed in Petri dishes containing PDA medium (Potato Dextrose Agar - 1.0% glucose, 1.5% agar in potato broth, added 500 mg amoxicillin) and for the isolation of bacteria, serial dilutions of the samples (10-1, 10-2, 10-3 and 10-4; 50 µL) were performed and spread into Petri dishes containing NA medium (Nutrient Agar - 0.3% meat extract, 0.5% peptone and 1.5% agar, added 1000 U nystatin). Plates were incubated at 28 ºC for 7 days and at 37 ºC for 48h for the isolation of fungi and bacteria, respectively. After growth of microorganisms, isolates were preserved in 20% glycerol at -80 °C (ultra preservation method). Thirty-three filamentous fungi were isolated, being 16 from coral, 15 from sea urchin, 1 from algae and 1 from biofilm samples. On the other hand, 66 bacteria were isolated, being 41 from biofilm, 27 from algae and 8 from barnacle samples. The microorganism will be analyzed for the macro and microscopic characteristics and for their potential on industrial and bioremediation process. The results suggest that samples derived from the marine environment, including macroorganisms and sediments may be considered promising sources in obtaining a great microbial community including filamentous fungi and bacteria.