EVALUATION OF MYCELIAL GROWTH AND SPORULATION OF DIFFERENT ISOLATES OF Trichoderma sp.

The fungus of the genus Trichoderma belonging to the family Hypocreceae, is a natural component found in soil that have an important ecological function besides partipating as a natural biofungicida. Some lineages are used to control phytopathogens and are used to promote the plant growth due their versatility of action, such as parasitism, antibiosis and competition, besides the acting as inductors of resistance of plants against diseases for the production of enzymes, organic acids, phosphate solubilization and others. Among the filamentous fungi, those of the genus Trichoderma are distinguished by their high capacity of enzymatic production, therefore, they distinguished in the degradation of cellulose making them one of the most studied and researcher fungi in laboratory in Brazil. Knowing about the importance of these fungi have the objective about this study was to evaluate the micelial growth and sporulation of twenty-two different isolates of Trichoderma sp. In this work, the samples were collected in several regions of Brazil and isolated with the intention of studying them as a potency of enzymatic production, mainly the cellulase. Were analyzed the rate of mycelial growth rate (IVCM) and the percentage of sporulation of conidia in BDA (potato –dextrose-agar) and using six replicates for each sample. For mycelial growth, the evaluation was performed by measuring, daily, of the diameter of the colonies. For the sporulation percentage it was adopted a time of five days after the total plate filling by the fungus. In this way, the reading of conidia production was made in a Neubauer chamber. All the samples presented mycelial growth and percentage of sporulation of conidia. Having a great variability between the isolates for the evaluated variables, IVCM and also for sporulation and conidial germination capacity. The Trichoderma isolates number four, five, six, eight and twenty were those that presented higher IVCM, having samples number four and five the significantly different from the other samples. However, for the percentage of germination and conidia production, the isolates did not behave in the same way, and there were significant differences. The treatment that obtained the most statistical significance was the sample of number five comparing with the others.