ENZIMATIC POTENTIAL OF ACTINOBACTERIA ISOLATED FROM CAATINGA

Among the enzymes of biotechnological importance are: amylase, carboxymethylcellulose (CMCase) and xylanase for use in various agricultural and industrial processes. Therefore, the enzyme’s market moves billions of dollars a year, and the search for new sources tends to intensify, focused on clean and renewable sources such as the microorganisms. The isolation of microorganisms from environments of water stress, it is presented as an alternative to obtain strains with biotechnological potential. The aim of this work was to evaluate the enzymatic potential of four strains of actinobacteria isolated from rhizosphere of the Catingueira (Caesalpinia pyramidalis); a native plant to the Caatinga of the Brazilian Northeast, opposite to the production of the enzymes: amylase, carboxymethylcellulose and xylanase. The strains were grown on ISP-3 and incubated at 37 ° C for 7 days. After this period, agar blocks (9 mm) of the cultures were transferred to the inducing media, starch (1%), carboxymethylcellulose (1%) and xylan (1%) and incubated at 37 ° C for 48 hours. To reveal enzymatic degradation for xylanase and CMCase, 10 mL of a Congo Red (0,1%) solution was sprinkled, the dishes were incubated at 40 ° C for 10 minutes, and washed with NaCl (1M). And for the amylase revelation, sublimated iodine was used for halos obtainment. The expression of the results was calculated by the enzymatic index, which is the relation between the diameter of the halo and the diameter of the colony, being considered those tests with IE ≥ 2 as valid for the qualitative tests. In the amylase assays, only strain 2 had IE ≥2, with a result of 2.2. In the CMCase tests all strains showed carboxymethylcellulase activity with IE ranging from 2.3 to 3.2. The xylanase activities showed just the strain 4 with xylanolytic activity with average IE of 2.3. With the results obtained, a biotechnological potential of these strains isolated from Caatinga was evidenced and that they are promising in the production of hydrolytic enzymes. Among the enzymes of biotechnological importance are: amylase, carboxymethylcellulose (CMCase) and xylanase for use in various agricultural and industrial processes. Therefore, the enzyme’s market moves billions of dollars a year, and the search for new sources tends to intensify, focused on clean and renewable sources such as the microorganisms. The isolation of microorganisms from environments of water stress, it is presented as an alternative to obtain strains with biotechnological potential. The aim of this work was to evaluate the enzymatic potential of four strains of actinobacteria isolated from rhizosphere of the Catingueira (Caesalpinia pyramidalis); a native plant to the Caatinga of the Brazilian Northeast, opposite to the production of the enzymes: amylase, carboxymethylcellulose and xylanase. The strains were grown on ISP-3 and incubated at 37 ° C for 7 days. After this period, agar blocks (9 mm) of the cultures were transferred to the inducing media, starch (1%), carboxymethylcellulose (1%) and xylan (1%) and incubated at 37 ° C for 48 hours. To reveal enzymatic degradation for xylanase and CMCase, 10 mL of a Congo Red (0,1%) solution was sprinkled, the dishes were incubated at 40 ° C for 10 minutes, and washed with NaCl (1M). And for the amylase revelation, sublimated iodine was used for halos obtainment. The expression of the results was calculated by the enzymatic index, which is the relation between the diameter of the halo and the diameter of the colony, being considered those tests with IE ≥ 2 as valid for the qualitative tests. In the amylase assays, only strain 2 had IE ≥2, with a result of 2.2. In the CMCase tests all strains showed carboxymethylcellulase activity with IE ranging from 2.3 to 3.2. The xylanase activities showed just the strain 4 with xylanolytic activity with average IE of 2.3. With the results obtained, a biotechnological potential of these strains isolated from Caatinga was evidenced and that they are promising in the production of hydrolytic enzymes. Programa de Pós-Graduação em Biologia de fungos, Universidade Federal de Pernambuco1, Programa de Pós-Graduação em Bioquímica e Fisiologia / Universidade Federal de Pernambuco2, Curso de Licenciatura em Ciências Biológicas/Universidade Federal de Pernambuco3, Departamento de Antibióticos/Universidade Federal de Pernambuco4 e Universidade Federal da Paraiba5. Among the enzymes of biotechnological importance are: amylase, carboxymethylcellulose (CMCase) and xylanase for use in various agricultural and industrial processes. Therefore, the enzyme’s market moves billions of dollars a year, and the search for new sources tends to intensify, focused on clean and renewable sources such as the microorganisms. The isolation of microorganisms from environments of water stress, it is presented as an alternative to obtain strains with biotechnological potential. The aim of this work was to evaluate the enzymatic potential of four strains of actinobacteria isolated from rhizosphere of the Catingueira (Caesalpinia pyramidalis); a native plant to the Caatinga of the Brazilian Northeast, opposite to the production of the enzymes: amylase, carboxymethylcellulose and xylanase. The strains were grown on ISP-3 and incubated at 37 ° C for 7 days. After this period, agar blocks (9 mm) of the cultures were transferred to the inducing media, starch (1%), carboxymethylcellulose (1%) and xylan (1%) and incubated at 37 ° C for 48 hours. To reveal enzymatic degradation for xylanase and CMCase, 10 mL of a Congo Red (0,1%) solution was sprinkled, the dishes were incubated at 40 ° C for 10 minutes, and washed with NaCl (1M). And for the amylase revelation, sublimated iodine was used for halos obtainment. The expression of the results was calculated by the enzymatic index, which is the relation between the diameter of the halo and the diameter of the colony, being considered those tests with IE ≥ 2 as valid for the qualitative tests. In the amylase assays, only strain 2 had IE ≥2, with a result of 2.2. In the CMCase tests all strains showed carboxymethylcellulase activity with IE ranging from 2.3 to 3.2. The xylanase activities showed just the strain 4 with xylanolytic activity with average IE of 2.3. With the results obtained, a biotechnological potential of these strains isolated from Caatinga was evidenced and that they are promising in the production of hydrolytic enzymes. Programa de Pós-Graduação em Biologia de fungos, Universidade Federal de Pernambuco1, Programa de Pós-Graduação em Bioquímica e Fisiologia / Universidade Federal de Pernambuco2, Curso de Licenciatura em Ciências Biológicas/Universidade Federal de Pernambuco3, Departamento de Antibióticos/Universidade Federal de Pernambuco4 e Universidade Federal da Paraiba5. Among the enzymes of biotechnological importance are: amylase, carboxymethylcellulose (CMCase) and xylanase for use in various agricultural and industrial processes. Therefore, the enzyme’s market moves billions of dollars a year, and the search for new sources tends to intensify, focused on clean and renewable sources such as the microorganisms. The isolation of microorganisms from environments of water stress, it is presented as an alternative to obtain strains with biotechnological potential. The aim of this work was to evaluate the enzymatic potential of four strains of actinobacteria isolated from rhizosphere of the Catingueira (Caesalpinia pyramidalis); a native plant to the Caatinga of the Brazilian Northeast, opposite to the production of the enzymes: amylase, carboxymethylcellulose and xylanase. The strains were grown on ISP-3 and incubated at 37 ° C for 7 days. After this period, agar blocks (9 mm) of the cultures were transferred to the inducing media, starch (1%), carboxymethylcellulose (1%) and xylan (1%) and incubated at 37 ° C for 48 hours. To reveal enzymatic degradation for xylanase and CMCase, 10 mL of a Congo Red (0,1%) solution was sprinkled, the dishes were incubated at 40 ° C for 10 minutes, and washed with NaCl (1M). And for the amylase revelation, sublimated iodine was used for halos obtainment. The expression of the results was calculated by the enzymatic index, which is the relation between the diameter of the halo and the diameter of the colony, being considered those tests with IE ≥ 2 as valid for the qualitative tests. In the amylase assays, only strain 2 had IE ≥2, with a result of 2.2. In the CMCase tests all strains showed carboxymethylcellulase activity with IE ranging from 2.3 to 3.2. The xylanase activities showed just the strain 4 with xylanolytic activity with average IE of 2.3. With the results obtained, a biotechnological potential of these strains isolated from Caatinga was evidenced and that they are promising in the production of hydrolytic enzymes. Programa de Pós-Graduação em Biologia de fungos, Universidade Federal de Pernambuco1, Programa de Pós-Graduação em Bioquímica e Fisiologia / Universidade Federal de Pernambuco2, Curso de Licenciatura em Ciências Biológicas/Universidade Federal de Pernambuco3, Departamento de Antibióticos/Universidade Federal de Pernambuco4 e Universidade Federal da Paraiba5. Among the enzymes of biotechnological importance are: amylase, carboxymethylcellulose (CMCase) and xylanase for use in various agricultural and industrial processes. Therefore, the enzyme’s market moves billions of dollars a year, and the search for new sources tends to intensify, focused on clean and renewable sources such as the microorganisms. The isolation of microorganisms from environments of water stress, it is presented as an alternative to obtain strains with biotechnological potential. The aim of this work was to evaluate the enzymatic potential of four strains of actinobacteria isolated from rhizosphere of the Catingueira (Caesalpinia pyramidalis); a native plant to the Caatinga of the Brazilian Northeast, opposite to the production of the enzymes: amylase, carboxymethylcellulose and xylanase. The strains were grown on ISP-3 and incubated at 37 ° C for 7 days. After this period, agar blocks (9 mm) of the cultures were transferred to the inducing media, starch (1%), carboxymethylcellulose (1%) and xylan (1%) and incubated at 37 ° C for 48 hours. To reveal enzymatic degradation for xylanase and CMCase, 10 mL of a Congo Red (0,1%) solution was sprinkled, the dishes were incubated at 40 ° C for 10 minutes, and washed with NaCl (1M). And for the amylase revelation, sublimated iodine was used for halos obtainment. The expression of the results was calculated by the enzymatic index, which is the relation between the diameter of the halo and the diameter of the colony, being considered those tests with IE ≥ 2 as valid for the qualitative tests. In the amylase assays, only strain 2 had IE ≥2, with a result of 2.2. In the CMCase tests all strains showed carboxymethylcellulase activity with IE ranging from 2.3 to 3.2. The xylanase activities showed just the strain 4 with xylanolytic activity with average IE of 2.3. With the results obtained, a biotechnological potential of these strains isolated from Caatinga was evidenced and that they are promising in the production of hydrolytic enzymes. Among the enzymes of biotechnological importance are: amylase, carboxymethylcellulose (CMCase) and xylanase for use in various agricultural and industrial processes. Therefore, the enzyme’s market moves billions of dollars a year, and the search for new sources tends to intensify, focused on clean and renewable sources such as the microorganisms. The isolation of microorganisms from environments of water stress, it is presented as an alternative to obtain strains with biotechnological potential. The aim of this work was to evaluate the enzymatic potential of four strains of actinobacteria isolated from rhizosphere of the Catingueira (Caesalpinia pyramidalis); a native plant to the Caatinga of the Brazilian Northeast, opposite to the production of the enzymes: amylase, carboxymethylcellulose and xylanase. The strains were grown on ISP-3 and incubated at 37 ° C for 7 days. After this period, agar blocks (9 mm) of the cultures were transferred to the inducing media, starch (1%), carboxymethylcellulose (1%) and xylan (1%) and incubated at 37 ° C for 48 hours. To reveal enzymatic degradation for xylanase and CMCase, 10 mL of a Congo Red (0,1%) solution was sprinkled, the dishes were incubated at 40 ° C for 10 minutes, and washed with NaCl (1M). And for the amylase revelation, sublimated iodine was used for halos obtainment. The expression of the results was calculated by the enzymatic index, which is the relation between the diameter of the halo and the diameter of the colony, being considered those tests with IE ≥ 2 as valid for the qualitative tests. In the amylase assays, only strain 2 had IE ≥2, with a result of 2.2. In the CMCase tests all strains showed carboxymethylcellulase activity with IE ranging from 2.3 to 3.2. The xylanase activities showed just the strain 4 with xylanolytic activity with average IE of 2.3. With the results obtained, a biotechnological potential of these strains isolated from Caatinga was evidenced and that they are promising in the production of hydrolytic enzymes.