79093

EFFECTS OF CELECOXIB ON REACTIVE OXYGEN SPECIES PRODUCTION IN METASTASTIC MELANOMA CELLS

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The expression of Cyclooxygenase 2 (COX-2) has been studied in several cancers. Currently, we demonstrated the COX-2 expression in high metastatic melanomas. Other studies have reported the correlation of Reactive Oxygen Species (ROS) production and COX-2 overexpression. Thus, in the current study we evaluated the ROS production in B16F10 cells treated or not with a COX-2 inhibitor (celecoxib). In addition, these data were correlated with damage to the genetic material by expression of bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) and with the cellular viability assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT)-test. After 24 hours with celecoxib treatment, B16F10 cells demonstrated an altered morphology, with nuclear pyknosis and cytoplasmic degeneration, these aspects were compatible with are compatible with cell death process. The IC50 of this drug was 7.5 μM, in this dose, the treated groups demonstrated considerable reduction of the number of cells. A decrease in rates of cell proliferation and a significant reduction of ROS in celecoxib treated cells at their IC50 indicate that this drug has cytotoxic activity, reduces ROS production and consequently is able to reduce cell proliferation. We concluded that celecoxib is an indicated drug for the treatment of aggressive melanomas.