This work aimed to study the microencapsulation of anatto seed oil using inulins with two degree of polymerazation (DP), GR-inulin (DP ≥ 10) and HP-inulin (DP ≥ 23), as wall material. The microparticles obtained by freeze-drying (FD) were caracterized with respect to scanning electron microscopy (SEM), confocal scanning laser microscopy (CSLM), encapsulation efficiency (EE) and differential scanning calorimetry (DSC).