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Abstract

Eugenia involucrata DC. is a native plant from Southern Brazil with a large concentration of phenolic compounds (PC) in different parts of the plant. Our objective was to evaluate the ability of E. involucrata ethanolic extracts of the pulp and seed to inhibit α-amylase, α-glucosidase, and pancreatic lipase. To α-amylase inhibition was used concentrations in the range of PC from cherry pulp extract (0.03 - 1.80 µg.mL-1) or range of PC from seed extracts (0.09 - 46.24 µg.mL-1), or positive control acarbose (44.0 - 352.0 µg.mL-1). In the inhibition of α-glucosidase was used extracts of cherry pulp (PC: 0.11 - 1.10 µg.mL-1) or seeds (PC: 0.72 - 2.89 µg.mL-1), or positive control acarbose (1,075.0 - 2,1500.0 µg.mL-1). For pancreatic lipase inhibition was used concentrations range of PC from cherry pulp extract (0.28 - 2.20 µg.mL-1) or range of seed extract (0.29 - 14.44 µg.mL-1), or positive control orlistat (0.0 - 49.6 µg.mL-1). The pulp extract had greater inhibitory potency than seed extract, and this two extracts was more potent than acarbose to inhibit α-amylase (IC50: pulp 0.2 ± 0.0; seed 1,3 ± 0.2; acarbose 39.9 ± 3.9 µg.mL-1) and α-glucosidase (IC50: pulp 0.3 ± 0.0; seed 1.5 ± 0.0; acarbose 2019.0 ± 81.3 µg.mL-1). In lipase inhibition, a greater inhibitory potency was observed for orlistat (IC50 0.1 ± 0.0 µg.mL-1), whereas pulp (IC50 0.7 ± 0.0 µg.mL-1) and seed (IC50 0.6 ± 0.0 µg.mL-1) extract had lower inhibitory potency. Our results indicate that E. involucrata pulp and seed extracts have promising potential to inhibit the studied digestive enzymes, and the inhibition of these enzymes indicates an anti-diabetic and anti-obesogenic potential of the PC from E. involucrata pulp and seed extracts.

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Author

Franciele Aline Smaniotto

Olá, já realizamos, previamente, um ensaio piloto in vivo  com ratos wistar, onde avaliamos o efeito do extrato da polpa de E. involucrata em modelo de diabetes tipo 2. O extrato da polpa demonstrou reduzir os níveis de glicose sanguínea após o tratamento durante 21 dias.

Para o extrato da semente, fica um tanto inviável a realização de ensaios in vivo, pois o rendimento deste extrato é bem menor quando comparado ao da polpa da cereja.

Institutions
  • 1 Universidade Federal de Santa Maria / Centro de Ciências Rurais / Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
  • 2 Universidade Federal de Santa Maria
  • 3 Universidade Federal de Santa Maria / Centro de Ciências Rurais / Departamento de Tecnologia e Ciência dos Alimentos
  • 4 Universidade Federal de Santa Catarina
Track
  • Food and health (AS)
Keywords
Digestive enzymes; phenolic compounds; cherry-of-Rio-Grande