POTENTIAL IN THE AMYLASES PRODUCTION BY FILAMENTOUS FUNGUS ISOLATED FROM THE AMAZON BIOME AND ENZYME CHARACTERIZATION

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Detalhes
  • Tipo de apresentação: Pôster
  • Eixo temático: Bioquímica e Biotecnologia de Alimentos (BB)
  • Palavras chaves: starch; fungi; ENZYMATIC PRODUCTION;
  • 1 Universidade Estadual Paulista “Júlio de Mesquita Filho” - Campus Ilha Solteira
  • 2 Departamento de Fitotecnia, Tecnologia de Alimentos e Sócio Economia / Faculdade de Engenharia de Ilha Solteira / Universidade Estadual Paulista “Júlio de Mesquita Filho”

POTENTIAL IN THE AMYLASES PRODUCTION BY FILAMENTOUS FUNGUS ISOLATED FROM THE AMAZON BIOME AND ENZYME CHARACTERIZATION

Heloiza Ferreira Alves do Prado

Departamento de Fitotecnia, Tecnologia de Alimentos e Sócio Economia / Faculdade de Engenharia de Ilha Solteira / Universidade Estadual Paulista “Júlio de Mesquita Filho”

Resumo

Starch is one of the main reserve carbohydrates found in nature. Syrups are an important product in the food industry that are from starch hydrolysis. Starch hydrolysis can be performed by a pool of amylolytic enzymes, including alpha-amylase, beta-amylase, glucoamylase, among others. Microbial amylases meet their industrial application characteristics. The screening of filamentous fungi with potential for the production of amylolytic enzymes was accomplished in an area of the Amazon biome. Twenty-three fungal isolates were studied and the isolate named 22.3 showed potential in the production of amylase, using wheat bran as substrate, under solid state cultivation. Solid state cultivation was carried out in 250 mL Erlenmeyrs flasks. The determination of amylolytic activity was performed using the dinitrosalisylic acid (DNS) method with 0.5% soluble starch as the reaction substrate. Isolate 22.3 presented activity of 130.93 ± 7.9 U g 1 substrate, in 48 hours of cultivation. Productivity was evidenced within 48 hours of cultivation. The biochemical characterization of the amylase produced by isolate 22.3 showed stability pH between pH 3.0 to 11.0 and optimum pH at pH 5.5, showing a wide range of pH tolerance and an acidic amylase. Amylase was stable up to 40ºC and showed excellent activity at 55ºC.

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