Anais do Simpósio Latino Americano de Ciências de Alimentos
Anais do 14 SLACA - Simpósio Latino Americano de Ciência de Alimentos

Propolis is a resinous substance produced by bees through plant secretions, being a product of constituition determinated by the phytogeographic characteristics existing in the hive region. Due to its antioxidant, anti-inflammatory and antimicrobial characteristics, propolis has been widely used as a home remedie since ancient times, the medicinal use of propolis extracts can be dated up to 300b.C. The objective of this work was to evaluate the chemical profile of green propolis samples through the mass spectrometry with paper spray ambient ionization technique (PSMS). The green propolis samples were gathered by the cooperative of rural producers in the Bambuí-MG region and taken to the organic chemistry laboratory of UFSJ-CSL for analysis. The propolis was subjected to methanol extraction, centrifuged for 15 minutes, supernatant was extracted. For the PSMS analysis, 2 µL of extract and 40 µL of methanol were applied in a chromatographic paper cut in an equilateral triangle shape and attached to a tension fount, positioned in from of the Ion Trap LCQ Fleet type mass spectrometer. The spectra obtained through the PSMS technique were interpreted using the Xcalibur software and, subsequently, the identified ions were compared, through their mass/charge ratios, with compounds identified in the literature with the same mass/charge ratio. 7 compounds were identified in the positive mode, these being: benzoic acid, methyl-eugenol, cadinene, liquiritigenin, formononetin, medicarpine and vestitol. The presence of flavonoids indicates the antioxidant and anti-inflammatory action of the extract obtained, highlighting its potential for pharmacological use, while the presence of benzoic acid is linked to the antimicrobial action of the extract. Thus, this study demonstrates the ability to detect ions through paper spray ionization, as well as its practicality compared to other ambient ionization techniques. It also reinforces the exploitation potential of propolis in terms of its antioxidant, anti-inflammatory and antimicrobial properties.