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FIXED BED PERCOLATION EXTRACTION: A SIMPLE AND EFFECTIVE TECHNIQUE FOR OBTAINING PHENOLIC COMPOUNDS FROM BARBATIMÃO (Stryphnodendron adstringens (Mart.) Coville) BARK
Juliane Viganó
Faculdade de Engenharia de Alimentos / Universidade Estadual de Campinas
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Crie um tópicoStryphnodendron adstringens, also known as Barbatimão, is native tree from the Brazilian Cerrado rich in bioactive compounds and traditionally used as an anti-inflammatory, analgesic, and antiseptic agent. This work aimed to obtain extracts from barbatimão bark applying the conventional extraction techniques Soxhlet and fixed bed percolation. The extracts were evaluated in terms of global yield (wt.%) and total phenolic content (TPC) by the Folin-Denis method. Tukey test at a significance level of 5% was used to identify the difference between the extraction methods. Soxhlet extractions used ethanol and methanol as the solvent for 6 hours. The fixed bed percolation extractions were kinetically performed, assessing the effect of the solvents, ranging from water to pure ethanol (0, 50, and 100% ethanol, m/m), temperature (40, 55, and 70°C), and extraction time (5, 15, 30, 45, 60, 90, and 120 min). The results from Soxhlet demonstrated that ethanol increased the global yield (38.69 ± 0.06%) compared to methanol; however higher TPC was obtained with methanol (342 ± 14 mg TAE/g dry material). Regarding the fixed bed percolation extractions, the best performance condition in TPC (485 ± 10 mg TAE/g dry material) was 50% ethanol at 55°C in 90 min of extraction, obtaining global yield equal to 51 ± 5%. According to the results, all kinetics performed at fixed bed percolation showed a higher global yield than those achieved in Soxhlet. Furthermore, alcoholic mixtures concentration was more advantageous in obtaining higher amounts of TPC. Finally, according to the global yield and TPC results, the percolation time required to complete depletion of the raw material was 90 min for all extractions. The results presented in this work revealed fixed bed percolation as an accessible, cheap, and green extraction technique able to recover high TPC content from Barbatimão bark.
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