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EXTRACTION OF CYANIDIN-3-GLUCOSIDE FROM JABOTICABA (Myrciaria cauliflora) PEEL THROUGH PRESSURIZED WATER IN SEQUENTIAL REACTORS
Tiago Linhares Cruz Tabosa Barroso
Universidade Estadual de Campinas
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ANÁLISE DA SECAGEM DE AÇÚCAR PURGADO POR RADIAÇÃO INFRAVERMELHAJaboticaba peel has a high content of anthocyanins, a bioactive compound with many applications in the food and pharmaceutical industries. The extraction of anthocyanins from industrial by-products using eco-friendly technologies is a trending topic. The pressurized liquid extraction (PLE) uses a solvent, such as water, under high-pressure conditions, facilitating the extraction capacity of the solvent, and stands out for not using chemicals, fast time, and is environmentally safe. Sequential reactors allow for more prolonged contact of the extraction solvent with the material, expanding the yield in batch processes. Therefore, this study aimed to extract cyanidin-3-glucoside from jaboticaba peel through sequential pressurized water reactors. The process was operated with 10 g jaboticaba peel in each reactor (coded as R1 and R2), 15 MPa, 5 mL/min, and a kinetic time of 45 minutes. Two operating temperature conditions were evaluated: 60 °C in R1 and R2 (process A) and 60 °C in R1 and 135 °C in R2 (process B). Conventional extraction was performed at 100 RPM, 70 °C, 45 min, and the same solvent-to-feed mass ratio than in the sequential process (11.2), applying water acidified at pH 2 with acetic acid as the solvent. The cyanidin-3-glucoside was quantified by high-performance liquid chromatography with a photodiode array detector. The yield of cyanidin-3-glucoside was 0.94 mg/g, 0.18 mg/g, and 0.82 mg/g for processes A, B, and conventional extraction, respectively. The sequential process showed a favorable result over the conventional one. Furthermore, the highest extraction yield was obtained applying the lowest temperatures, which avoided the thermal degradation of anthocyanins. Finally, pressurized water extraction in two sequential reactors can be promising for recovering anthocyanins from jaboticaba by-products.
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