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APPLICATION OF ALCALASE® COMMERCIAL ENZYME FOR OBTAINING BIOACTIVE PEPTIDES FROM SOYBEAN BRAN
Thamara Thaiane da Silva Crozatti
Universidade Estadual de Maringá
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Crie um tópicoSoybean meal is a by-product obtained from soy processing and represents a potent protein source and a promising alternative for the development of foods rich in bioactive peptides with functional properties. Bioactive peptides are short-chain amino acid fragments that have one or more biologically significant activities in human metabolism. The objective of this study was to apply the biotechnological process of enzymatic hydrolysis with commercial Alcalase® protease to obtain bioactive di and tri-peptides from soybean meal. The hydrolysis was carried out for 4 h with 10 mg of Alcalase® enzyme (pH 8 at 55 °C) for 1% substrate (m/m). The crude protein content was determined by the Kjeldahl method and the antioxidant potential by the method of the radical DPPH and ABTS. The bioactive peptides of the hydrolysates were detected by the technique of mass spectrometry (LC-MS/MS) based on the neutral loss (NL) of 46 Da. The biological activities of the identified peptides were determined based on the BIOPEP-UWM database. The enzymatic hydrolysis performed on the soybean meal showed a crude protein content above 70%, which resulted in an average increase of 20% in the initial protein content of the substrate (49.31%). Enzymatic hydrolysis improved the antioxidant activity of soybean meal and resulted in 398.00 ± 0.22 for ABTS and 80.5 ± 0.36 for DPPH. The mass spectrometry technique detected 19 peptides (16 di and 03 and tri-peptides), which presented sequences of antioxidant, antihypertensive, and antidiabetic bioactivities. The data obtained in this research proved that enzymatic hydrolysis with commercial protease can be a promising alternative for obtaining bioactive peptides from soybean meal and, therefore, an interesting possibility for the development of functional products with different physiological effects.
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