EFFECT OF 3D PRINTED PLASTIC RUSHTON TURBINE AND STANDARD METAL TURBINE ON COMMERCIAL BAKERY SACCHAROMYCES CEREVISIAE GROWTH KINETICS

vol. 4, 2019 - 116626
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Resumo

Nowadays, 3D printing has become cheaper and available – 3D printer for less than US$ 200 are available on the market. The 3D printing process is revolutionizing several fields, such as medicine, construction, robotics, manufacturing, and several others. In bioprocessing, this technique has a vast potential (e.g., for prototyping) and have great potential to optimize the fermentation process. However, differences in the production process and the type of material used to construct bioreactor impeller the effect fermentation is unknow. This work aimed to compare a 3D printed impeller and standard metal impeller on commercial baker’s yeast (Fleischmann®) growth kinetics and sugar consumption (HPAEC-PAD), to evaluate if the 3D printed impeller causes differences in the fermentation process, using a NewBrunwsick BioFlo 310 (2L work volume) bioreactor. The 3D printed turbine was made in a FFF printer (Anet a8) using PLA plastic, and the parameter used was 0.2 mm layer height, three perimeters, and 50% infill. The fermentation occurred at 300 rpm, 1 vvm, 30 ºC for 24 hours. The kLa for the 3D and for the metal impellers were 0,578h-1 (±0,059) and 0,511h-1 (±0,054), respectively. There were no statistically significant differences among them by Tukey (p<0.01). Moreover, the growth kinetics and glucose consumption were also statistically similar (p<0.01) for both impellers. Our results show that the 3D printed impeller may be used to replace the conventional metal impeller, and suggests that 3D printing may be useful optimize the costs in fermentation processes.

Instituições
  • 1 Departamento de Ciência de Alimentos / Faculdade de Engenharia de Alimentos / Universidade Estadual de Campinas
Eixo Temático
  • 6. Bioquímica e Biotecnologia de Alimentos (BB)
Palavras-chave
3D Printing
fermentation
kLA