Anais do Simpósio Nacional de Bioprocessos
XXII National Bioprocesses Symposium (SINAFERM) XIII Enzymatic Hydrolysis of Biomass Symposium (SHEB)

Extraction of β-fructofuranosidase (FFase) from Aspergillus tamarii URM4634 produced by solid-state fermentation was performed by Aqueous Two-Phase Systems (ATPS) PEG-Citrate. The influence of variables PEG molar mass (MMPEG), PEG concentration (CPEG), citrate concentration (CCIT) and pH on FFase partition coefficient (K), activity yield (Y) and purification factor (PF) were evaluated by a 24-full factorial design. FFase partitioned preferentially to the PEG-rich phase and presenting high activity yield (146.83 %) in the condition: MMPEG = 8000 g/mol, CPEG = 24% (w/w), CCIT = 20% (w/w) and pH=8.0. Therefore, the results obtained indicate that the utilization of PEG-Citrate ATPS is a good technique for use as the first stage of FFase extraction.