EFFECT OF TEMPERATURE IN α-AMYLASE AND AMYLOGLUCOSIDASE PRODUCED FROM ENGLISH POTATO RESIDUE VIA FERMENTATION IN SOLID STATE

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Resumo

The objective of the present study was to use the English potato residue as a substrate of the solid state fermentation (SSF) of the fungus Aspergillus niger for the production of the enzymes alpha amylase and amyloglucosidase. A spores solution of concentration 109 was used. The fermentations were conducted at 30 °C in a bacteriological oven, with 35%, 60% and 75% moisture content, at times of 48, 72 and 96 hours. Fermentation was carried out with and without the presence of the inducer (1 g corn starch). The study variable was the optimum temperature, necessary for the inclusion of enzymes in industrial routes. The temperatures analyzed were 30, 40, 50, 60 and 70 °C. The highest α-amylase activity was at 30 ° C yielding 192.8 U / g, in the presence of the inducer the temperature with the best activity was 70 ° C yielding 258.8 U / g. Glucoamylase presented a lower temperature preference, 30 ° C without the inductor and 40 ° C with the presence of inductor, with an activity of 230.41 U / g and 313.81 U / g respectively.

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Instituições
  • 1 Departamento de Engenharia e Ciências Exatas / UNESA - Campos dos Goytacazes / Universidade Estácio de Sá
  • 2 Departamento de Engenharia Química / Instituto de Tecnologia / Universidade Federal Rural do Rio de Janeiro
  • 3 UNESA - Campos dos Goytacazes / Universidade Estácio de Sá
  • 4 Embrapa Agroindústria de Alimentos / Bolsista produtividade CNPq / EMBRAPA
  • 5 Empresa Brasileira de Pesquisa Agropecuária
Eixo Temático
  • 6. Processos Enzimáticos
Palavras-chave
Bioconversion fungal
Aspergillus niger
Solanum tuberosum L