Anais do Simpósio Nacional de Bioprocessos
XXII National Bioprocesses Symposium (SINAFERM) XIII Enzymatic Hydrolysis of Biomass Symposium (SHEB)

Fall armyworm (Spodoptera frugiperda) is a severe world crop pest and presents serious aggravations on its management, becoming a very relevant agricultural problem. The great economic and environmental expend caused by agrochemicals favors the emergence of biopesticides, such as the baculovirus group, a natural and specific insect controller. This strategy was successfully used before with in vivo production, however it’s a costly and laborious approach which requires a spacious infrastructure. With that in mind, baculovirus in vitro production and the better understanding of it could bring improvements on final price, quality and yield. Previous studies of our research group suggested that gas sparging could cause shear stress in Sf9 insect cell line, compromising SfMNPV baculovirus infection. In order to test that, we compared Wave bioreactor versus sparged STR (BioFlo 110) on cell kinetics, growth parameters and polyhedra production. The results show that cell concentration reaches higher values in Wave, therefore the polyhedra production is enhanced significantly. Besides that, Wave approach appears to generate more polyhedra particles per cell.