Adaptation of a genome-scale metabolic model to study Saccharomyces cerevisiae anaerobic metabolism

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Resumo

Yeasts will play an important role as platform microorganisms for the processes of conversion of sugars into fuel ethanol but also into other advanced fuels and chemicals. The increase of fermentation yields poses many scientific and technological challenges. Genome-scale metabolic models (GSM) emerge as a powerful tool for this task, mainly for the design of gene knockout strategies. However, the gene knockouts predictions depend on the accuracy of GSM fluxes prediction. Therefore, this contribution aims to adapt the GSM model Yeast 8.30 in order to accurately describe the anaerobic metabolism of Saccharomyces cerevisiae in the presence of glucose or xylose as the carbon source. Metabolic fluxes measurements of CEN.PK113-7D were used to adapt the model to anaerobic glucose metabolism, and experiments with different xylose consuming pathways were compared with in silico predictions. According to the analysis the GSM model Yeast 8.30 needs some adaptation in order to describe correctly the glucose metabolism and the xylose isomerase and reductase pathways behavior in anaerobic conditions. After the model adaptation it is ready to be used for gene knockouts strategies application.

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Instituições
  • 1 Departamento de Engenharia Química / Escola Politécnica / Universidade de São Paulo
Eixo Temático
  • 1. Biologia Molecular e Biologia de Sistemas Aplicadas a Bioprocessos
Palavras-chave
Metabolic Engineering
Saccharomyces cerevisiae
Genome-Scale Metabolic Models
Xylose
Chemostat cultivation