Lipases are ubiquitous enzymes with industrial potential since they have advantages such as biocatalysts, high catalytic activity, high stability, environmental friendliness, and remarkable chemical, enantio and regioselectivity. It is desirable to concentrate the protein solution in order to protein separation can be faster during subsequent purification steps. The objective of this work was purified a Metschnikowia sp. lipase from Antarctic environment using ammonium sulfate precitipation and the results showed this method was capable to recorver over than 100% of inicial enzyme activity with 15.34 fold purification. It can be concluded ammonium sulfate precipitation with 80% saturation was able to separate the product of interest from other proteins, decrease and concentrate the enzyme in lower volume and increasing the enzymatic activity of lipase in study.