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PRODUCTION OF GLUCONIC ACID FROM GLUCOSE CATALYZED BY CROSS-LINKED AGGREGATES OF GLUCOSE OXIDASE AND CATALASE IN A BUBBLE COLUMN REACTOR

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Cross-linked aggregates of glucose oxidase (GOD) from Aspergillus niger were prepared, evaluating the influence of the type of precipitant agent and cross-linker concentration, as well as bovine serum albumin as feeder protein on the GOD activity. The yield of immobilization was 56%. A combination of CLEAs of GOD and catalase was used in the gluconic acid production from glucose solution (26 g.L-1, 40 oC, pH 6.0) in a pneumatic reactor, yielding around 80% conversion.