Polyhydroxyalkanoates (PHA) are biodegradable polyesters synthesized by a variety of bacteria, which can are accessible by the biotechnological conversion of cheap sources. The purpose of this study was to evaluate the production of PHA in medium containing crude glycerol from the biodiesel their molecular mass and thermal properties. Cupriavidus necator IPT027 was grown in saline medium containing CGB or glucose (180 rpm, 35 ° C, 72 h). The tested bacteria were able to produce a higher concentration of PHA in a medium containing CGB (0.53 g L-1) than in medium containing glucose (0.28 g L-1). Thus, the discrepancy in the PHA production values may be associated with the bacterial species, source carbon nature, and process conditions. In addition, results of molecular mass (630.72 10³ g mol-1), Tm (176.63 °C), Tonset (251.05 °C) and Tdecomp (296.18 °C) higher than the values obtained from glucose. Therefore, the use of CGB for PHA production is promising.