58871

FAST AND EFFICIENT GENETIC MODIFICATION OF HUMAN CELLS UNDER SUSPENSION SERUM-FREE CONDITIONS FOR RECOMBINANT PROTEIN PRODUCTION: BY TRANSIENT TRANSFECTION OR TRANSDUCTION?

Favorite this paper Contact the authors

Human cell lines have attracted great interest because they are capable of producing glycosylated proteins that are more similar to native human proteins, thereby reducing the potential for immune responses. Despite the great potential, some promising human cell lines have not been extensively exploited for recombinant protein production, especially under serum-free suspension conditions. This work aims to evaluate two strategies to genetically modify the human cell lines Huh-7, HKB-11, SK-Hep-1 to further established an efficient platform for recombinant protein production. The results obtained so far, indicate that genetic modification of the cells by lentiviral transduction rather than transient transfection seems to be the best alternative to rapidly generate high-yield recombinant cells for protein production under serum-free suspension conditions. Recombinant erythropoietin-producing cells generated by this methodology presented high expression levels.