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Eugenyl isobutyrate is an ester derived from eugenol, the major component of clove essential oil. Eugenol esters has been studied due to their antimicrobial and antioxidant properties. The aim of this work was to verify the antimicrobial activity of eugenyl isobutyrate in pathogenic microorganisms. Eugenyl isobutyrate was obtained by esterification reaction, using isobutyric anhydride and eugenol molar ratio 2:1, with the enzymatic catalyst Novozym 435 (10% in relation to the total weight of the substrate) in batch mode, for 72 hours at 70 °C. The ester presented antimicrobial activity, against the microorganisms Salmonela sp. Salmonella enterica and Staphylococcus aureus, the concentration that showed the greatest inhibition halo in all the microorganisms tested was 0.50 mg.mL-1.