Desenvolvimento de genossensor empregando nanotubos de carbono e Poli-L-Lisina para a detecção da Hepatite C

Electrodes modified with nanomaterials associated with polymer are of great interest to develop electrochemical sensors. Among the materials used, the graphene oxide (GO) linked to Polythionin (PTh) has been gaining prominence due to its physical and chemical properties. The detection of Hydrogen peroxide (H2O2) is an important tool in food and environmental monitoring, diagnosis, and biological studies (1).This study aims to develop an electrochemical platform to detect H2O2 using PTh/GO in Phosphate Buffer Solution (PBS). The platform was formed by electropolymerization using a solution composed by 1 mM thionin with 0,5 mg/mL of GO in PBS. It was applied Ciclic voltammogram (CV) by a window potencial from -0.2 to 1.4 V, 50 v/s scan rate and 10mV of step during 20 cycles. It was used 5 mL of PBS as an indicator for electrochemical characterization of the electrode surface before and after electropolymerization by PTh/GO. At the end of this study, some concentration of Hydrogen Peroxide were tested on the platform in order to establish its detection limit (figure 1). Thus, this platform developed can allow the detection of hydrogen peroxide quickly and reliably through CV.