Nanopartículas superparamagnéticas tioladas como potenciais veículos biológicos

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Detalhes
  • Tipo de apresentação: e-Pôster
  • Eixo temático: Química Inorgânica - INO
  • Palavras chaves: NANOPARTICLES; iron oxide nanoparticles; Superparamagnetic; Biological Applications; citotoxicity;
  • 1 Universidade Federal de São Paulo
  • 2 Faculdade de Medicina do ABC / Faculdade de Medicina do ABC
  • 3 Universidade Federal do ABC
  • 4 Faculdade de Medicina do ABC

Nanopartículas superparamagnéticas tioladas como potenciais veículos biológicos

Tatiane Britos

Universidade Federal de São Paulo

Resumo

Superparamagnetic iron oxide nanoparticles (SPIONs), such as magnetite (Fe3O4), are known for their biocompatibility and applications in several areas of medicine1. The present research is focused on the synthesis, characterization, and biological application of SPIONs. Magnetite nanoparticles (Fe3O4 NPs) were synthesized by co-precipitation of iron salts and coated with biocompatible molecules such as L-cysteine (CYS)2 and Glutathione (GSH), making thioled-nanoparticles as Fe3O4-CYS and Fe3O4-GSH NPs, respectively. The physicochemical, structural, and magnetic properties were characterized by different experimental techniques, such as X-ray diffraction (XRD), Fourier transforms infrared (FTIR), and ultraviolet-visible (UV-vis) spectroscopies, SQUID magnetic measurements, and dynamic light scattering (DLS). The results showed that these nanoparticles have a mean diameter about of 15 nm, at a solid-state and good size for biological applications in aqueous dispersion, the average hydrodynamic sizes of these NPs were about 80 nm and has a superparamagnetic behavior at room temperature and the residual magnetization and coercive force were found to be zero. The titration with 5,5′-dithiobis (2-nitrobenzoic acid) molecule (DTBN) was used to quantify free thiol (-SH) groups on the surface of nanoparticles, we found a value of (0.122 ± 0.06) mmol.g−1 of -SH in Cys-Fe3O4. To evaluate their applicability in nanomedicine we evaluated their toxicity using Balb/C mice. Taken together the results suggest that coating magnetite nanoparticles with CYS lead to the formation of a stable aqueous dispersion of thiolated superparamagnetic nanoparticles synthesis was well succeeded which shows great potential as biomedical vehicle. 

1 P.S. Haddad, T.N. Britos, L.M. Li, L.D.S. Li. Journal of Physics. Conference Series, v. 617, p. 012002, (2015).
2 T.N. Britos, C.E. Castro, B.M. Bertassoli, G. Petri, F.L.A. Fonseca, F.F. Ferreira, P.S. Haddad, Mater. Sci. Eng. C 99, 171 (2019).

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Autor

Tatiane Britos

Agradeço muito e peço desculpas pela falta do vídeo, por gentileza entre novamente que agora ele está disponível. Aproveito para convidá-la à assistir a apresentação do meu trabalho de forma estendida na sexta-feira (26) às 10:30 hs na sessão de Inorgânica. Estou à disposição. Abraços, Tatiane Britos.