The use of pesticides has been associated with the drastic decline of bee populations in several countries. Honey bee species such as Apis mellifera are of utmost importance because of their vital ecological duty as pollinators of wild plants and crops. Consequently, scientific advisory bodies have standardized in vivo protocols to access acute toxicity of chemicals in honey bees. In view of the costly and time-consuming characteristics of experimental assays, we developed an innovative artificial intelligence-based web portal to access acute toxicity of chemicals in honey bees. Initially, we have collected and curated the largest publicly available dataset of compounds with acute contact and oral toxicity for A. mellifera. Then, 40 machine learning (ML) models were built using contact and oral datasets to distinguish toxic (LD₅₀ ≤11 µg/bee) vs. non-toxic (LD₅₀ >11 µg/bee) compounds. The model built using FeatMorgan_2 fingerprint + Random Forest demonstrated the best external predictivity among all other models developed for acute contact toxicity (ACC = 92%; SE = 83%; SP = 96%; and MCC = 81%). The model built using MACCS fingerprints + Support Vector Machine shown the best external predictivity among all other models developed for acute oral toxicity (ACC = 91%; SE = 93%; SP = 93%; and MCC = 82%). These models are freely available through the BeeToxAI web portal at http://beetoxai.labmol.com.br/. The BeeToxAI (Figure 1) allows users for fast screening of compounds. After drawing or importing a molecule and submitting it to a prediction, the user will receive in few seconds the predicted outcomes for acute contact and oral toxicities. These predictions are followed by probability estimates, which are useful for estimating the confidence of predicted outcomes, the applicability domain estimates, and mechanistic interpretation of prediction using contribution maps (atoms with positive and negative contributions for toxicity). In summary, BeeToxAI can be used as a reliable alternative to animal tests for predicting acute toxicity of chemicals in honey bees.