MAPPING NANOBIOINTERFACE: SYNCHROTRON ENHANCED CIRCULAR DICHORISM INSIIGHTS

CEDRO is a beamline at Sirius dedicated to Circular Dichroism (CD) spectroscopy in the ultraviolet region. CD spectroscopy involves the differential absorption of circularly polarized light, distinguishing between left and right polarization, by optically active entities such as chiral materials. It serves as a valuable tool for studying the structure of chiral molecules, including biomolecules like proteins and nucleic acids. By utilizing synchrotron radiation instead of conventional lamps, the spectroscopy technique is enhanced and referred to as synchrotron radiation circular dichroism (SRCD). This transition offers advantages, including higher intensity and the ability to record CD spectra over an extended energy range. The CD technique has been widely used in the analysis of the secondary structure of proteins, but is still little explored in the nanobiointerface. This work presents the possibility of studies in this broad area and the advantages that a synchrotron source brings to this frontier. The layout and status of the beamline will be presented, as well as the first results and future developments aimed at meeting the varying requirements of different users.