UHPLC method development to analyze the extraction parameters optimization of secondary metabolite from the leaves of Amburana cearensis (Allemão) A. C. Sm. and determination of phenolic and flavonoid content.

Vol 9, 2023 - 167226
Poster
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Abstract

The aim of the following work was to develop a UHPLC-DAD chromatographic method
to analyze the hydroethanolic leaves extract of A. cearensis, evaluate the extraction parameters
using box Behnken design to obtain a higher level of the secondary metabolites and determine
the total phenolic and flavonoid content. The extraction condition 20 mg/mL (w/v), ethanol 62%,
43.8 minutes for the ultrasonic extraction leaded to the highest yield and secondary metabolites
quantity.

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Institutions
  • 1 Universidade Federal do Rio Grande do Norte
Track
  • 1. Biodiversity and Natural Products
Keywords
Amburana cearensis; UHPLC method development; Box-Behnken design; phenolic compounds