A metabolomics approach to evaluate the biosynthetic potential of in vitro micropropagated Melia azedarach L.

We established an in vitro culture of Melia azedarach through micropropagation and evaluated the plantlets biosynthetic potential. LC-MS-based metabolomics was applied to generate information on natural products composition. Our findings indicate limonoid production from the nimbolinin and salannin classes and a series of phenylpropanoids. This is the first time nimbolinin-related limonoids have been produced in vitro, opening a venue for the biotechnological application of such compounds.