Subacute preclinical toxicity of bioproduct (pulp, bark and endocarp) of Buriti lyophilized.

Preclinical tests of toxicity of natural products are carried out to characterize potential adverse effects and to provide initial estimates of the safety margin for humans, especially in new food ingredients, pharmaceuticals and Nutraceuticals. Buriti is a fruit of excellent nutritional value and functional potential. This study aimed to determine the effects of subacute administration (28 days) on biochemical, hematological and histological parameters, as well as locomotor activity and motor coordination of mice treated with aqueous extracts of pulp, bark and Buriti endocarp in Swiss mice, female, weighing between 25-35 g. Repeated doses were administered for 28 days in groups (n = 7) of 300 mg/Kg and 600 mg/Kg, in addition to the negative control group (water, V.O. 1mL/100g; n = 7). To evaluate the behavioral tests, a positive control group was used (Diazepam 1 mg/Kg, I.P., n = 5). Behavioral evaluation and hippocratic screening were performed by observing the animals up to 3h after daily administration, notifying signs of toxicity and death. Every 2 days, weight, feed intake and water were quantified up to 28 days. There was no alteration in the hippocratic screening, nor statistical difference in the weight of the organs between treated and control groups. There were also no macroscopic and histological alterations (heart, kidneys, lung, liver, spleen, stomach and brain). In the biochemical profile, there was a reduction in glucose, triglycerides and total cholesterol for all groups under treatment. HDL-C significantly reduced (P < 0.05) only in the group treated with bark extract at the highest administered dose (600 mg/Kg). Therefore, extracts from the pulp, bark and endocarp of buriti fruit did not present subacute toxicity on rodents, but it is suggested a possible action in the glycidic and lipid control of mice.