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The use of byproducts in the diet of sheep is an alternative to reduce the cost of production of meat of these animals, but the meat science seeks the highest degree of consumer satisfaction, which is gradually prioritizing the nutritional value of food. Based on this background, studies involving the impact of the use of byproducts in the nutritional quality of lamb become essential. The objective of this study was to evaluate the chemical composition and cholesterol of muscle Longissimus lumborum of Ile de France lambs fed with biodiesel co-products. The muscles were originated from 40 male lambs uncastrated fed four diets: D0 = forage + concentrate; DP = forage + concentrate + 20% peanut meal; DG = forage + concentrate + 25% glycerin; DPG = forage + concentrate + 10% peanut meal + 12.5% glycerin, the treatments were so characterized. The experimental design was completely randomized. Data were submitted to analysis of variance were compared by Tukey test (5%). We used the computer program SAS (2003). There was no difference (P>0.05) for moisture, protein and fat content, the ash content was higher (P <0.05) in DP (1.12%) and lowest in DG (0.98%), DC, and DPG were similar the others (1.06% and 1.08%), the highest dietary minerals value with peanut meal, as well as the aminoacids profile this byproduct can have corroborate with higher ash content meat. The cholesterol content (mg / 100g flesh) was lower DPG (110.88), higher for DP (118.86), however DC, and DG were similar to the other treatments (113.52 and 111.34), these results may be related to the lipid profile of the byproducts used, as the meat fat was not influenced by diet. Concluding that the use of the peanut meal diet promotes lambs increased ash content and cholesterol in meat.