PURIFIED QUERCETIN SUPPLEMENTATION NO SHOWED POSITIVE EFFECT ON OBESITY PARAMETER IN RATS.

PURIFIED QUERCETIN SUPPLEMENTATION NO SHOWED POSITIVE EFFECT ON OBESITY PARAMETER IN RATS.
¹Reis, L. S. L. S.; ²Lenquiste, S. A.; 3Maia, J. P. C.; 4 Fagiani, M. A. B.; 5Tsugigushi, L. K.; 6Mello, F.A.
1, 2, 3, 4, 5, 6 University of Western São Paulo (UNOESTE) – Presidente Prudente / São Paulo.
E-mail: [email protected]; [email protected]; [email protected]; [email protected]; [email protected]; [email protected].
Quercetin is a phenolic compound naturally present in foods, especially purple onion and other purple vegetables. Antioxidant, anti-inflammatory and antiobesogenic effects have been attributed to this bioactive compound in studies with food source and purified quercetin. We evaluated the effect of purified quercetin supplementation in rats feed obesogenic diet on weight gain, fat accumulation and insulin sensibility. Therefore, 70 Wistar rats were divided in seven groups: normal control (NC) fed with Supralab® rodent commercial diet; obese control (OC) fed with high-fat fructose diet composed by commercial diet added of 30% of lard and supplemented with 20% fructose; normal quercetin supplemented (NQ10) fed with NC diet and supplemented with 10mg quercetin for day; normal quercetin supplemented (NQ20) fed with NC diet and supplemented with 20mg quercetin for day; obese quercetin supplemented (OQ10) fed with OC diet and supplemented with 10mg quercetin for day; obese quercetin supplemented (OQ20) fed with OC diet and supplemented with 20mg quercetin for day. Quercetin supplementation was done by guar gum bait added with purified quercetin (95%) and offered daily for rats. Diet intake and weight gain were monitored daily and weekly, respectively. Intraperitoneal Glucose Tolerance Test (iGTT) and Intraperitoneal Insulin Tolerance Test (kITT) were realized. After 16 weeks, animals were euthanized and liver, epidydimal adipose tissue (EAT), mesenteric adipose tissue (MAT) and retroperitoneal adipose tissue (RAT) were weighted. Serum glucose was measured by enzymatic test. We done ANOVA variance test with post-hoc Tukey test with limit of significance set at P < 0.05. Diet intake, weight gain and serum glucose was not different among groups. Liver and adipose tissues (EAT, MAT, RAT) weight also were was not different among groups. Is possible that isolated quercetin intake do not be so effective in health parameters as food source of this bioactive compound, according with some previous references. Thus, purified quercetin supplementation not showed positive effect on experimental model testing. Propose news studies for evaluated the isolated quercetin supplementation effects in comparison with food source of this bioactive compound in other experimental model.
Key words: purified quercetin, obesity, insulin resistance.